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When employing IEM, i5 sequences has to be entered into these software program in the ahead orientation in all situations and for all instrument kinds.
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For the i5 index, evaluate colour balance using the ahead orientation for MiSeq operates and MiniSeq operates with rapid reagents, and also the reverse-complement orientation for all other instruments.
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If managing in handbook manner and utilizing bcl2fastq or BCL Change, or uploading the sample sheet to BaseSpace being a handbook manner operate and attaching a sample sheet, enter the i5 sequence within the forward route.
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